Drive BioTech
Bis-Tris Precast Gel
Bis-Tris Precast Gel
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Bis-Tris Precast Gel
|
CAT. |
Description |
|
PG4110 |
Bis-Tris Precast Gel, 4-20%, 10 wells, 10 gels/Box |
|
PG4112 |
Bis-Tris Precast Gel, 4-20%, 12 wells, 10 gels/Box |
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PG4113 |
Bis-Tris Precast Gel, 4-20%, 15 wells, 10 gels/Box |
Introduction
The Bis-Tris SDS-PAGE Gel system offers effective electrophoretic separation and better band resolution over a wide size range of proteins. Bis-Tris Precast Gels are specifically designed to work with the MOPS SDS running buffer (Cat: PS201). Typical migration patterns of protein on gels of different concentrations are listed in Figure 1.
Figure 1. Protein Separation using Bis-Tris Precast Gels with MOPS SDS running buffer.
Loading Volume Suggestion
|
Well type |
Recommended Loading Volume |
Maximum Loading Volume |
Maximum Protein Load |
|
10-well |
Less than 25 μl |
50 μl |
0.5 μg/band |
|
12-well |
Less than 20 μl |
40 μl |
0.5 μg/band |
|
15-well |
Less than 15 μl |
30 μl |
0.5 μg/band |
Instruction for use
- Running buffer preparation: Dissolve one bag of MOPS SDS Running Buffer (CAT: PS201) in 1 liter deionized water to make 1× Gel running buffer.
- Remove Bis-Tris Precast Gel from the package, peel off the pink sealing tape at the bottom of the gel cassette, and gently remove the comb in the direction in Figure 2.
- Install the gel cassette onto an electrophoresis tank. Ensure the short side of the cassette facing inside.
When using Bio-Rad electrophoresis tanks (Bio-Rad Mini-PROTEAN® II and 3 and Bio-Rad Mini-PROTEAN® Tetra System), reverse the green gasket to its flat back side (see Figure 3). If not done, the buffer will leak into the anode (outer) chamber.
Figure 2. Remove Sealing Tape and comb from Gel Cassette.
Figure 3. Flipping the rubber gasket of the electrophoresis tank.
- Fill the tank with 1× running buffer. Ensure the liquid level of the inner chamber is higher than the short side of the cassette. For the outside chamber, the buffer should not exceed the long side of the cassette. Otherwise, a current short circuit will happen, and the electrophoresis will be abnormal.
- After Loading samples, cover the electrophoresis tank and plug the electrical leads into the power supply.
- Run the gel at a constant voltage of ~160 V until the dye front reaches the bottom of the gel cassette. Run time can vary depending on the gel percentage, running buffer, and equipment used.
- When electrophoresis is finished, remove the gel cassette from the gel tank. Insert the Gel Cassette Opener into the gap between the two plates at one of the three contact points along each side of the cassette (see Figure 4). Repeat for all six contact points of the cassette until the two plates are separated.
- Gently detach the gel from the cassette and perform downstream experiments (gel staining, western blot, ).
Storage
Gels can be stored at 4°C for one year.